The original Polish method of collagen hydration seemed to be defined and its temperature issues unsolvable. Collagen would break down depending on the temperature (read more in this article here)
But we persevered.
Piotr Pakuła, a young successful businessman and a passionate biochemist concentrated on the biochemistry of proteins. This inspired Dr Jan Czarnecki, a researcher into technologies obtaining pharmaceutical components and creator of many drugs, to conduct research. In 2010, at a laboratory in Finepharm Jelenia Gora, Poland. He extracted collagen from fish skins with an almost ideal biological purity. It was not, however, transformed into a marketable product because the problem of breaking it down by colagenase enzymes was not yet solved. This is a highly interesting subject for medicine, especially in orthopaedics. Simultaneously, Dr Czarnecki’s team was looking for a form of fish native collagen which, while maintaining its biological activity, would at the same time increase its heat resistance.
Eventually, such a product was invented!
Dr Czarnecki assumed that maintaining the triple helix conformation in the collagen gel is not a necessary condition for the biological activity of its peptides. He was also the first to scientifically prove that in order for the peptides of the Polish collagen gel to be absorbed, the triple helices must break down on the skin into single spirals (secondary collagen). Ultimately, while on the skin, collagen spirals have to break down into smaller protein chunks to be able to penetrate the skin. Czarnecki’s brilliant idea was to perform a controlled dissimilation of triple spirals into single spirals, while maintaining the gel form of the hydrate.
In 2012 it became possible using a physical, and not chemical method. Utilising kinetics, pressure, and temperature, while maintaining extraordinary precision which is only possible in a perfectly equipped laboratory (formerly “Jelfa” from Jelenia Góra), Dr Czarnecki’s team performed the first completely controlled breakdown of the triple helices into single helices.